Engineering new cell functionalities on thin films

Researchers from Mainz have developed a method to engineer new functionalities into cells and better understand eukaryotic cell functions

25 August 2021

Numerous processes occur inside living cells, from DNA replication and repair to protein synthesis and recycling. In order to organize this plethora of reactions, they must be separated in three-dimensional space. One way eukaryotic cells do this is by extruding a piece of membrane to form a membrane-enclosed space – an organelle – in which specific functions can take place. Alternatively, the cell can also segregate molecules into distinct areas, so-called membraneless organelles, through phase separation, a phenomenon similar to the separation of vinegar and oil in a salad dressing. Such membraneless organelles have advantages: as they are not separated from the rest of the cell by a membrane barrier, large molecules can get in and out more easily. Membrane-enclosed organelles therefore operate like separate "rooms" in a cell, while membraneless organelles operate like different "corners" of the same room.

Unique properties – and a better understanding

One of the most important processes in the cell is protein synthesis, where the RNA code is translated into a protein code containing the blueprint for making proteins. These codes are like the languages of the cell. If an organelle could be engineered and dedicated to translate the RNA code in new ways, i.e., use a different language, the functions of the resulting protein could also be changed, endowing it with unique properties that could be used, for example, to switch its functions on or off or to allow the protein to be visualized in living cells.

In 2019, Professor Edward Lemke and his research team succeeded in creating an artificial membraneless organelle that translated the RNA code using a new code, or language, without interfering with RNA translation in the rest of the cell. Now Lemke and a student from his lab, Dr. Christopher Reinkemeier, have further built on this success by creating film-like organelles that can be used to subdivide cell processes into even smaller spaces. "The biggest gain is that we were able to create extremely small reaction spaces.This way we can have several of them in a cell at the same time," explained Lemke, Professor of Synthetic Biophysics at Johannes Gutenberg University Mainz and Adjunct Director at IMB. "We have converted the large 3D organelles into 2D organelles on a membrane surface and can even run complicated biochemical reactions in these thin layers." Using these thinner organelles, the same cell can now translate the RNA code into three different languages – thus creating three different proteins – in different "corners" of the same "room", without the translations interfering with each other. This means that the same protein can now have three different functions, depending on in which "corner" it was made.

This novel method not only allows scientists to engineer proteins with unique functions, but also helps them to better understand how eukaryotic cell functions evolve. "We can find out more about how complicated functions occur in the membrane space, what unique functionalities the membrane has, and what special reaction spaces are created there when you concentrate proteins using 2D phase separation," said Reinkemeier. "Through engineering these film-like organelles, we can also better understand how nature uses such mechanisms to create proteins with new functions."

The research results have been published in the article "Dual film-like organelles enable spatial separation of orthogonal eukaryotic translation" in the journal Cell.